Recombinant Human Interferon γ/IFN-γ (E. coli)

產(chǎn)品描述：Recombinant Human Interferon gamma is produced by our E.coli expression system and the target gene encoding Gln24-Gln166 is expressed.

別名：Interferon γ蛋白, IFN-γ蛋白;Interferon Gamma, IFN-Gamma, Immune Interferon, IFNG

來源：Escherichia coli.

形態(tài)：Lyophilized from a 0.2 μm filtered solution of 20mM PB, 300mM NaCl, 5%Mannitol, 0.1%Tween80, 5%Trehalose, pH6.0.

序列：MQDPYVKEAENLKKYFNAGHSDVADNGTLFLGILKNWKEESDRKIMQSQIVSFYFKLFKNFKDDQ SIQKSVETIKEDMNVKFFNSNKKKRDDFEKLTNYSVTDLNVQRKAIHELIQVMAELSPAAKTGKR KRSQMLFRGRRASQ

純度：Greater than 95% as determined by reducing SDS-PAGE.

內(nèi)毒素水平：Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

活性：Measured by a cytotoxicity assay using HT-29 cells.The ED50 for this effect is 17 pg/mL.

溶解方法：Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

保存方法：Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

背景說明：IFNγ is the major interferon produced by mitogenically or antigenically stimulated lymphocytes. It is structurally different from type I interferon and its major activity is immunoregulation. It has been implicated in the expression of class II histocompatibility antigens in cells that do not normally produce them, leading to autoimmune disease. Interferon gamma is produced mainly byT-cells and natural killer cells activated by antigens, mitogens, or alloantigens. It is produced by lymphocytes expressing the surface antigens CD4 and CD8. IFNγ synthesis is induced by IL-2, FGF-basic, and EGF.