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| 品牌 | absin | 規格 | 10ug |
|---|---|---|---|
| 供貨周期 | 一周 |
| 概述 | |
|---|---|
| 描述 | Recombinant Human Interleukin-2 is produced by our E.coli expression system and the target gene encoding Pro22-Thr153 is expressed. |
| 別名 | Interleukin-2, IL-2, T-Cell Growth Factor, TCGF, Aldesleukin, IL2 |
| 形態 | Lyophilized from a 0.2 μm filtered solution of 20mM HAc-NaAc, 150mM NaCl, pH 4.0. |
| 來源 | E.coli |
| 氨基酸序列 | MPTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKAKHLQCLEEELKP LEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNRWITFCQSIIS TLT |
| 內毒素水平 | Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
| Accession # | P60568 |
| 性能 | |
|---|---|
| 背景 | Recombinant Human Interleukin-2 is a highly purified protein with a molecular weight of approximay 15,300 Daltons. The chemical name is des-alanyl-1, serine-125 Human Interleukin-2. It is produced by recombinant DNA technology using a genetically engineered E. coli strain containing an analog of the human interleukin-2 gene. Genetic engineering techniques were used to modify the Human IL-2 gene, and the resulting expression clone encodes a modified Human IL-2. This recombinant form differs from native Interleukin-2 in following ways: 1) it is not glycosylated; 2) the molecule has no N-terminal alanine; 3) the molecule has serine substituted for cysteine at amino acid position 125; 4) the aggregation state of molecule is likely to be different from that of native IL-2. |
| 溶解方法 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 保存方法 | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
| 純度 | Greater than 95% as determined by reducing SDS-PAGE. |
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