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Recombinant Mouse IFN-γ

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詳細介紹

品牌	absin	規格	20ug
供貨周期	一周

概述
種屬	Murine
形態	Lyophilized from a 0.2 µm filtered solution in PBS, pH 7.4, containing 5 % trehalose.
來源	Escherichia coli.
氨基酸序列	HGTVIESLES LNNYFNSSGI DVEEKSLFLD IWRNWQKDGD MKILQSQIIS FYLRLFEVLK DNQAISNNIS VIESHLITTF FSNSKAKKDA FMSIAKFEVN NPQVQRQAFN ELIRVVHQLL PESSLRKRKR SRC
內毒素水平	Less than 1 EU/µg of rMuIFN-γ as determined by LAL method.
活性	Fully biologically active when compared to standard. The ED₅₀ as determined by an anti-viral assay using murine L929 cells infected with encephalomyocarditis (EMC) virus is less than 0.8 ng/ml, corresponding to a specific activity of > 1.3 × 10⁶ IU/mg.
Accession #	P01580
預期分子量	Approximay 15.5 kDa, a single non-glycosylated polypeptide chain containing 133 amino acids.

性能
溶解方法	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
保存方法	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
純度	> 96 % by SDS-PAGE and HPLC analyses.
Gene IDs	15978

參考文獻
1. Pennino D, Bhavsar PK, Effner R, et al. 2012. J Allergy Clin Immunol, <br/>2. Hibi M, Hachimura S, Ise W, et al. 2003. Cytotechnology, 43: 49-55.<br/>3. Wang H, Ruan Z, Wang Y, et al. 2008. Mol Immunol, 45: 1548-56.<br/>4. Kopinski P, Przybylski G, Jarzemska A, et al. 2007. Pol Merkur Lekarski, 23: 15-21.

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