Recombinant Rhesus Macaque GM-CSF（2ug）

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詳細介紹

品牌	absin	貨號	abs00962
規格	2ug	供貨周期	一周

概述
種屬	Rhesus Macaque
形態	Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
來源	Escherichia coli.
氨基酸序列	APARSPSPGT QPWEHVNAIQ EARRLLNLSR DTAAEMNKTV EVVSEMFDLQ EPSCLQTRLE LYKQGLQGSL TKLKGPLTMM ASHYKQHCPP TPETSCATQI ITFQSFKENL KDFLLVIPFD CWEPVQE
內毒素水平	Less than 1 EU/μg of rRhGM-CSF as determined by LAL method.
活性	Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using human TF-1 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 10⁷ IU/mg.
Accession #	Q9GL44
預期分子量	Approximay 14.4 kDa, a single non-glycosylated polypeptide chain containing 127 amino acids.

性能
溶解方法	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
保存方法	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
純度	> 98 % by SDS-PAGE and HPLC analyses.

參考文獻
1. Wang JM, Chen ZG, Colotta F, et al. 1988. Behring Inst Mitt: 270-3. 2. 1989. N Engl J Med, 320: 253-4. 3. Nissen-Druey C. 1989. Nouv Rev Fr Hematol, 31: 99-101. 4. Eager RandNemunaitis J. 2005. Mol Ther, 12: 18-27. 5. Tran T, Fernandes DJ, Schuliga M, et al. 2005. Br J Pharmacol, 145: 123-31.

參考文獻

1. Wang JM, Chen ZG, Colotta F, et al. 1988. Behring Inst Mitt: 270-3.

2. 1989. N Engl J Med, 320: 253-4.

3. Nissen-Druey C. 1989. Nouv Rev Fr Hematol, 31: 99-101.

4. Eager RandNemunaitis J. 2005. Mol Ther, 12: 18-27.

5. Tran T, Fernandes DJ, Schuliga M, et al. 2005. Br J Pharmacol, 145: 123-31.

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