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                首頁 > 產(chǎn)品中心 > 細(xì)胞生物學(xué) > 重組蛋白 > abs00875Recombinant Human aFGF

                Recombinant Human aFGF

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                二抗、標(biāo)簽抗體、對(duì)照抗體;WB相關(guān):ECL發(fā)光液、預(yù)染marker、預(yù)制膠;IHC相關(guān):二抗試劑盒、組化筆;IP/CoIP試劑盒;細(xì)胞因子、信號(hào)通路調(diào)節(jié)劑;血清、BSA、蛋白酶K、CTB、TTX、CEE;凋亡試劑盒、呼吸爆發(fā)試劑盒;動(dòng)植物提取物...

                • 產(chǎn)品型號(hào):abs00875
                • 廠商性質(zhì):生產(chǎn)廠家
                • 更新時(shí)間:2025-06-11
                • 訪  問  量:1365

                詳細(xì)介紹

                品牌absin貨號(hào)abs00875
                規(guī)格10ug供貨周期一周
                概述
                種屬Human
                形態(tài)Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
                來源Escherichia coli.
                氨基酸序列MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D
                內(nèi)毒素水平Less than 1 EU/µg of Recombinant HumanaFGF as determined by LAL method.
                活性Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg in the presence of 10 μg/ml of heparin.
                Accession #P05230
                預(yù)期分子量Approximay 16.0 kDa, a single non-glycosylated polypeptide chain containing 141 amino acids.
                性能
                溶解方法We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
                保存方法This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
                純度> 95 % by SDS-PAGE and HPLC analyses.
                Gene IDs2246
                參考文獻(xiàn)

                1. Dionne CA, Crumley G, Bellot F, et al. 1990. EMBO J. 9:2685-92.

                2. Jaye M, Howk R, Burgess W, et al. 1986. Science. 233:541-5.

                3. Armelin HA. 1973. Proc Natl Acad Sci U S A. 70:2702-6.

                4. Gospodarowicz D. 1974. Nature. 249:123-7.

                5. Zhang X, Ibrahimi OA, Olsen SK, et al. 2006. J Biol Chem. 281:15694-700.

                6. Ornitz DM, Xu J, Colvin JS, et al. 1996. J Biol Chem. 271:15292-7.

                7. Eswarakumar VP, Lax I, Schlessinger J. 2005. Cytokine Growth Factor Rev. 16:139-49.

                8. Landriscina M, Bagala C, Mandinova A, et al. 2001. J Biol Chem. 276:25549-57.

                9. Fernandez IS, Cuevas P, Angulo J, et al. 2010. J Biol Chem. 285:11714-29.

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